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Protein

Translation initiation factor IF-2, chloroplastic

Gene

At1g17220

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi508 – 515GTPBy similarity8
Nucleotide bindingi558 – 562GTPBy similarity5
Nucleotide bindingi612 – 615GTPBy similarity4

GO - Molecular functioni

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor IF-2, chloroplastic
Gene namesi
Ordered Locus Names:At1g17220
ORF Names:F20D23.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G17220
TAIRilocus:2020427 AT1G17220

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 63ChloroplastSequence analysisAdd BLAST63
ChainiPRO_000001447764 – 1026Translation initiation factor IF-2, chloroplasticAdd BLAST963

Proteomic databases

PaxDbiQ9SHI1
PRIDEiQ9SHI1

PTM databases

iPTMnetiQ9SHI1

Expressioni

Gene expression databases

ExpressionAtlasiQ9SHI1 baseline and differential
GenevisibleiQ9SHI1 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G17220.1

Structurei

3D structure databases

ProteinModelPortaliQ9SHI1
SMRiQ9SHI1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini499 – 672tr-type GAdd BLAST174

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni508 – 515G1By similarity8
Regioni533 – 537G2By similarity5
Regioni558 – 561G3By similarity4
Regioni612 – 615G4By similarity4
Regioni648 – 650G5By similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1145 Eukaryota
COG0532 LUCA
HOGENOMiHOG000076906
InParanoidiQ9SHI1
KOiK02519
OMAiGNPQACV
OrthoDBiEOG093604R2

Family and domain databases

Gene3Di3.40.50.10050, 1 hit
HAMAPiMF_00100_B IF_2_B, 1 hit
InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR000178 TF_IF2_bacterial-like
IPR015760 TIF_IF2
IPR023115 TIF_IF2_dom3
IPR036925 TIF_IF2_dom3_sf
IPR009000 Transl_B-barrel_sf
PANTHERiPTHR43381 PTHR43381, 1 hit
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF11987 IF-2, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 2 hits
SSF52156 SSF52156, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00487 IF-2, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51722 G_TR_2, 1 hit
PS01176 IF2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SHI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSMLVLVGT MPSLASLVSL GGACASVSGT SSSDASYALV KRVSLSRRSV
60 70 80 90 100
KGTKKWLCRY SVSSSTTTTT ADFIADQNNN SVSIDSNSFR GSKDGDDSEV
110 120 130 140 150
VLKQTPKPVL KPPVARVERG LGVNTAPWSK DLSNGGKFDG EEERNKVIES
160 170 180 190 200
LGEVLDKAEK LEIPKPGNKE GGEAVKPSQP SANSSNSRNG SYANASDGGT
210 220 230 240 250
RKTKTMKSVW RKGDAVAAVQ KVVKESPKIF NRGVQTEPRT REEGEVNAKA
260 270 280 290 300
GTPLAPPQPP FRPQPPVRPQ PMLQGKPMVA PPVKKSPILK DLGMAAKPLV
310 320 330 340 350
SEEVDSSVKS KERKPILVDK FASKKKGVDP AASQAVLAPT KPGKGPPSNK
360 370 380 390 400
FRVEHRNKKN ASASPRRRIV AEDDGDDDAS ISRSGRKGRK WSKASRKAVR
410 420 430 440 450
LQAAKDAAPV KAEILEVEEE GMSIEDLAYN LAIGEGDILG YLYSKGIRPD
460 470 480 490 500
GVHTLDREMV KMICRDYDVE VLDADSVKVE EMAKKRQTFD EEDLDKLEDR
510 520 530 540 550
PPVITIMGHV DHGKTTLLDY IRKSKVAASE AGGITQGIGA YKVSVPVDGK
560 570 580 590 600
LQSCVFLDTP GHEAFGAMRA RGARVTDIAI IVVAADDGIR PQTNEAIAHA
610 620 630 640 650
KAAAVPIVIA INKIDKEGAS PDRVMQELSS IGLMPEDWGG DVPMVQISAL
660 670 680 690 700
KGENVDDLLE TVMLVAELQE LKANPHRNAK GIVIEAGLDK AKGPFATFIV
710 720 730 740 750
QKGTLKRGDV VVCGEAFGKV RALFDHSGER VDEAGPSIPV QVIGLNNVPI
760 770 780 790 800
AGDEFEIVSS LDVAREMAEA RAVSLRDERI SAKAGDGKVT LSSLASAVSA
810 820 830 840 850
KKMSGLDLHQ LNIILKVDVQ GSIEAVRQAL QVLPQENVTL KFLLQATGDV
860 870 880 890 900
SNSDVDLASA SEAIVFGFNV KASGSVKKAA ENKGVEIRLY RVIYELIDDV
910 920 930 940 950
RNAMEGLLES VEEQIPIGSA EVRATFSSGS GRVAGCMVNE GKFVKDCGIR
960 970 980 990 1000
VVRKGKTVHV GVLDSLKRVK ENVKEVSAGL ECGIGMDDYD DWIEGDIIEA
1010 1020
FNAVQKRRTL EEASASMSAA IEEAGV
Length:1,026
Mass (Da):109,747
Last modified:May 31, 2011 - v2
Checksum:i9D2E8620C2E7BB1D
GO

Sequence cautioni

The sequence AAD50011 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti779R → G in AAN46761 (PubMed:14593172).Curated1
Sequence conflicti779R → G in AAK32930 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007651 Genomic DNA Translation: AAD50011.1 Different initiation.
CP002684 Genomic DNA Translation: AEE29560.1
AF367343 mRNA Translation: AAK32930.1
BT001007 mRNA Translation: AAN46761.1
AK317746 mRNA Translation: BAH20402.1
PIRiD86308
RefSeqiNP_173165.1, NM_101583.4
UniGeneiAt.18936

Genome annotation databases

EnsemblPlantsiAT1G17220.1; AT1G17220.1; AT1G17220
GeneIDi838293
GrameneiAT1G17220.1; AT1G17220.1; AT1G17220
KEGGiath:AT1G17220

Similar proteinsi

Entry informationi

Entry nameiIF2C_ARATH
AccessioniPrimary (citable) accession number: Q9SHI1
Secondary accession number(s): B9DI35, Q9ASQ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 31, 2011
Last modified: April 25, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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